Below are the steps of solid-phase peptide synthesis:
1)The protecting groups of the amino acids are removed.
2)The peptide is released from the solid support resin.
3)An incoming amino acid is activated at the carboxyl group by DCC and added to the column.
4)Fmoc is removed by treatment with a base and the C-terminal amino acid is attached to the resin molecule.
5)The resin bound C-terminal amino acid and the incoming N-terminal amino acid are coupled.What is the appropriate order of these steps?
A)3,5,4,2,1
B)1,4,2,5,3
C)4,3,5,1,2
D)2,4,5,3,1

Question

Quizplus · Accepted Answer

The appropriate order of steps in solid-phase peptide synthesis is:
1) The protecting groups of the amino acids are removed.
2) Fmoc is removed by treatment with a base, and the C-terminal amino acid is attached to the resin molecule.
3) An incoming amino acid is activated at the carboxyl group by DCC and added to the column.
4) The resin-bound C-terminal amino acid and the incoming N-terminal amino acid are coupled.
5) The peptide is released from the solid support resin.
Therefore, the correct order of steps is 4, 3, 5, 1, 2, which is provided in option C.

Below Are the Steps of Solid-Phase Peptide Synthesis