Most secondary antibodies used in Western blots are covalently linked to the enzyme
A) luciferase.
B) horseradish peroxidase.
C) catalase.
D) hydrolase.

Question

Quizplus · Accepted Answer

Horseradish peroxidase (HRP) is commonly used as the enzyme linked to secondary antibodies in Western blotting. HRP can catalyze the reaction that produces a detectable signal (by converting a substrate such as luminol into a chemiluminescent product) when it is exposed to the appropriate detection reagents. This allows for the visualization of the protein of interest on the blot.

Most Secondary Antibodies Used in Western Blots Are Covalently Linked