Below are the steps of a Western blot.What is the correct order?
1)The membrane is incubated with the secondary antibody.
2)The membrane is incubated with the primary antibody.
3)The membrane is washed with a blocking solution.
4)Proteins are transferred to a nitrocellulose membrane.
5)Protein extracts are separated by gel electrophoresis.
A)5,4,3,2,1
B)4,5,2,3,1
C)3,4,5,1,2
D)2,5,4,3,1

Question

Quizplus · Accepted Answer

The correct order of steps in a Western blot is: proteins are first separated by gel electrophoresis (5), then transferred to a membrane (4), the membrane is washed with a blocking solution (3), incubated with the primary antibody (2), and finally with the secondary antibody (1).

Below Are the Steps of a Western Blot