One common way to characterize glycans is to
A) digest the glycan into fragments using trypsin and chymotrypsin.
B) use affinity chromatography to recognize the different sugars.
C) incubate fluorescently labeled glycoproteins with lectin.
D) use ELISA to determine the structure.

Question

Quizplus · Accepted Answer

Incubating fluorescently labeled glycoproteins with lectin allows for specific recognition and binding of certain glycan structures based on their sugar composition. This can then be observed using fluorescent microscopy or flow cytometry, providing information on the glycan composition of the sample. Digesting the glycan or using ELISA may not provide as specific information, and while affinity chromatography can also recognize sugars, it may not be as accurate or sensitive as lectin binding.

One Common Way to Characterize Glycans Is to