Which technique provides information about the arrangement of sugars in a glycan fraction?
A) glycan arrays
B) MALDI-TOF
C) mass spectrometry
D) high-performance liquid chromatography

Question

Quizplus · Accepted Answer

High-performance liquid chromatography (HPLC) is a technique commonly used to separate and analyze complex mixtures. It can also be used to analyze the arrangement of sugars in a glycan fraction by separating them based on their size, charge, and chemical properties. By comparing the retention times of different glycans in the HPLC chromatogram, information about their arrangement and composition can be obtained. Glycan arrays, MALDI-TOF, and mass spectrometry are also useful techniques for analyzing glycans, but they do not provide specific information about the arrangement of sugars in a glycan fraction.

Which Technique Provides Information About the Arrangement of Sugars in a Glycan