What is the function of the RNA primer during DNA synthesis?
A)DNA polymerase requires a 3' hydroxyl to initiate synthesis.
B)The primer helps the replication machinery find the origin of replication.
C)The primer is required for cleavage of the DNA to generate a loose end.
D)The primer signals DNA polymerase to terminate synthesis.

Question

Quizplus · Accepted Answer

During DNA synthesis, DNA polymerase requires a 3' hydroxyl group to initiate synthesis. However, DNA does not have a 3' hydroxyl group available for the polymerase to attach to, so an RNA primer with a 3' hydroxyl group is synthesized by primase to initiate DNA synthesis. Once the primer is in place, DNA polymerase can attach and elongate the DNA strand.

What Is the Function of the RNA Primer During DNA