Why is Escherichia coli DNA polymerase not very useful for PCR?
A)E.coli DNA polymerase cannot proofread during replication.
B)E.coli DNA polymerase only amplifies circular DNA.
C)E.coli DNA polymerase is heat labile.
D)E.coli DNA polymerase is too slow.

Question

Quizplus · Accepted Answer

E.coli DNA polymerase is heat labile, meaning it will denature at the high temperatures required for PCR. PCR typically requires a thermostable polymerase, such as Taq polymerase, that can withstand the repeated heating and cooling cycles.

Why Is Escherichia Coli DNA Polymerase Not Very Useful for PCR