DNase I protection assays or DNA footprinting is useful forـــــــــــــــــــــــ .
A)determining the start of transcription
B)determining the location of a DNA binding sequence
C)preventing contamination of RNA in primer extension samples
D)none of the above

Question

Quizplus · Accepted Answer

DNase I protection assays or DNA footprinting can be used to determine the location of a DNA binding sequence. It involves treating the DNA with DNase I enzyme, which will cleave the DNA at random locations unless it is protected by a protein bound to the DNA. By analyzing the resulting pattern of cleavage, researchers can determine the location and size of the protein-DNA complex. This information can be used to identify the specific DNA sequence that the protein binds to, allowing for further study of the protein's function in gene regulation.

DNase I Protection Assays or DNA Footprinting Is Useful Forـــــــــــــــــــــــ