How might the potential binding of a protein to a specific fragment of DNA be investigated?
A)primer extension
B)western blotting
C)gel mobility shift assays
D)isoelectric focusing

Question

Quizplus · Accepted Answer

Gel mobility shift (or EMSA) assays are commonly used to investigate the binding of a protein to a specific fragment of DNA. In this assay, labeled DNA fragments are mixed with the protein of interest and run on a gel. If the protein binds to the DNA, the resulting complex will migrate more slowly through the gel than the free DNA fragment, leading to a shift in the band pattern. This method is relatively simple and can provide information about the specificity and affinity of the protein-DNA interaction. The other options (primer extension, western blotting, and isoelectric focusing) are not appropriate for investigating protein-DNA interactions.

How Might the Potential Binding of a Protein to a Specific