The following methods that are used to determine the HIV viral load in a newly diagnosed patient. Which uses signal amplification?

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bDNA (branched DNA) uses signal amplification to detect and quantify HIV RNA. This method involves the hybridization of RNA to target-specific probes, followed by enzymatic amplification of signal to generate chemiluminescent signal. NASBA (nucleic acid sequence-based amplification) and RT-PCR (reverse transcriptase-polymerase chain reaction) both involve amplification of viral RNA/DNA, but they use different mechanisms (enzymatic and PCR-based, respectively). Sequencing is a method that determines the order of nucleotides in a DNA/RNA molecule and is not typically used for quantitative HIV viral load testing.

The Following Methods That Are Used to Determine the HIV