Question 1

Which of the following targets are used to type molds?

Accepted Answer

ITS (Internal Transcribed Spacer) regions are commonly used for typing molds and other fungi due to their variability between species, which helps in accurate identification.

Question 2

Which is a flow cytometry test?

Accepted Answer

HPV OncoTect is a flow cytometry test used to identify and quantify the presence of HPV (Human Papillomavirus) in cells, which can be an indicator of the risk of developing cervical cancer.

Question 3

Which virus does not share sequence homology with the others?

Accepted Answer

JC, BK, and SV40 are all polyomaviruses, while HCV is a flavivirus. Therefore, HCV does not share sequence homology with the others.

Question 4

The following methods that are used to determine the HIV viral load in a newly diagnosed patient. Which uses signal amplification?

Accepted Answer

bDNA (branched DNA) uses signal amplification to detect and quantify HIV RNA. This method involves the hybridization of RNA to target-specific probes, followed by enzymatic amplification of signal to generate chemiluminescent signal. NASBA (nucleic acid sequence-based amplification) and RT-PCR (reverse transcriptase-polymerase chain reaction) both involve amplification of viral RNA/DNA, but they use different mechanisms (enzymatic and PCR-based, respectively). Sequencing is a method that determines the order of nucleotides in a DNA/RNA molecule and is not typically used for quantitative HIV viral load testing.

Question 5

What characteristic of parasites complicates molecular testing?

Accepted Answer

Parasites often have tough outer layers or protective membranes that can resist normal methods of DNA extraction and isolation, making it difficult to obtain sufficient quantities of DNA for molecular testing. This can result in false negatives or inconclusive results.

Quiz 12: Detection and Identification of Microorganisms

Which of the following targets are used to type molds?

Which is a flow cytometry test?

Which virus does not share sequence homology with the others?

The following methods that are used to determine the HIV viral load in a newly diagnosed patient. Which uses signal amplification?

What characteristic of parasites complicates molecular testing?