Which of the following PCR controls ensures that the enzyme is active, the buffer is optimal, and the primers are priming the correct target sequence and must have a PCR product detected in order to be valid?

Question

Quizplus · Accepted Answer

The positive control is a sample that contains the target DNA sequence and should generate a PCR product. It serves as a control to ensure that the PCR reagents (enzyme, buffer, and primers) are functioning correctly. If the positive control fails to generate a product, it indicates a problem with the PCR reaction itself. Therefore, a positive control is necessary to validate the PCR reaction.

Which of the Following PCR Controls Ensures That the Enzyme