SYBR Green fluorescence is detectable at which stage of the PCR amplification process?

Question

Quizplus · Accepted Answer

SYBR Green fluorescence is detectable during primer annealing and extension in the PCR amplification process. This is when the double-stranded DNA is being synthesized from the primers, and SYBR Green binds to the newly synthesized DNA strands to emit fluorescence. SYBR Green can also bind to any non-specific amplification products or primer-dimer formations, leading to false positive signals.

SYBR Green Fluorescence Is Detectable at Which Stage of the PCR