In Maxam-Gilbert sequencing, co-migrating bands that are present in lanes from the tube treated with hydrazine (T or C) and the tube treated with hydrazine plus salt (T) are read as which of the following nucleotides?

Question

Quizplus · Accepted Answer

Co-migrating bands in Maxam-Gilbert sequencing are read as the nucleotide that is complementary to the one that was removed by the chemical treatment. Hydrazine treatment removes guanine (G) and xanthine, while hydrazine plus salt removes adenine (A), cytosine (C), and thymine (T). Therefore, co-migrating bands in lanes from the tube treated with hydrazine (T or C) and the tube treated with hydrazine plus salt (T) would indicate the presence of thymine (T) nucleotides.

In Maxam-Gilbert Sequencing, Co-Migrating Bands That Are Present in Lanes