Which of the following methods used to detect mutations is a sequencing (polymerization)-based method?

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Dideoxy DNA sequencing, also known as Sanger sequencing, is a polymerization-based method that uses modified nucleotides (dideoxynucleotides) to terminate DNA chain elongation, resulting in a set of fragments of different sizes that can be visualized on a gel or sequenced using automated methods. Restriction fragment length polymorphisms (RFLPs) and single-strand conformation polymorphisms (SSCPs) are based on changes in DNA fragment size or shape, respectively, that result from mutations, but do not involve sequencing per se. The Invader assay is a type of hybridization-based method that detects single nucleotide polymorphisms (SNPs) by detecting changes in probe fluorescence upon allele-specific cleavage of target DNA.

Which of the Following Methods Used to Detect Mutations Is