Which of the Following Describes the Procedure of the Autohemolysis

Which of the following describes the procedure of the autohemolysis test?

A) Incubation of patient RBCs with a series of graded hypotonic salt solutions
B) Sterile incubation of patient RBCs in their own plasma for 48 hours at 37°C
C) Sterile incubation of patient RBCs in their own plasma for 24 hours at 25°C
D) Sterile incubation of patient RBCs in their own plasma for 72 hours at 56°C
E) None of the above