The presence of polychromatophilic macrocytes in a treated megaloblastic anemia is due to:

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Polychromatophilic macrocytes are immature RBCs that still contain RNA and are stained with both acid and basic dyes. They are typically seen in reticulocytosis, which is an increase in immature RBCs in response to anemia or blood loss. In treated megaloblastic anemia, these polychromatophilic macrocytes are a sign of effective treatment and recovery.

The Presence of Polychromatophilic Macrocytes in a Treated Megaloblastic Anemia