You are designing an experiment that requires a protein to be exposed to high heat yet remain functional.You find, however, that the protein has lost its function on returning to its normal physiological temperature.You determine that the loss of function is due to an error in refolding of the protein.To remedy this, you could

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Adding a chaperone can help with the correct refolding of the protein by providing a protective environment for the protein to fold correctly. Exposing the protein to high temperature multiple times may cause more damage to the protein, and changing the peptide backbone or increasing the pH may alter the protein structure and lead to further loss of function. Mercaptoethanol can disrupt disulfide bonds, which may not be suitable for all proteins. Therefore, adding a chaperone is the best choice in this scenario.

You Are Designing an Experiment That Requires a Protein to Be