Which of the following would not be required for determining the sequence of a 400 bp DNA fragment by the high-throughput sequencing method?
A) The four dNTPs (dATP, dCTP, dGTP, dTTP)
B) DNA polymerase
C) A short, artificially synthesized primer
D) A bacterial artificial chromosome
E) Heat-denatured DNA fragments

Question

Quizplus · Accepted Answer

A bacterial artificial chromosome (BAC) is not required for high-throughput sequencing. BACs are used in molecular biology to clone and manipulate large pieces of DNA, but they are not needed for sequencing a 400 bp DNA fragment. The other choices, such as dNTPs, DNA polymerase, a primer, and denatured DNA fragments, are all necessary components of high-throughput sequencing.

Which of the Following Would Not Be Required for Determining