If the universal adapter sequence is 5′-GTCATTGCTTGCAATGTT-3′, which universal primer should be used for DNA sequencing?
A) 5′-GTCATTGCTTGCAATGTT-3′
B) 5′-TTGTAACGTTCGTTACTG-3′
C) 5′-CAGTAACGAACGTTACAA-3′
D) 5′-CCTGAATTGGTCGTACAA-3′
E) 5′-AACATGCTGGTTAAGTCC-3′

Question

Quizplus · Accepted Answer

The universal primer that should be used for DNA sequencing is complementary to the universal adapter sequence. Choice C, 5′-CAGTAACGAACGTTACAA-3′, is the reverse complement of the universal adapter sequence and would be appropriate for DNA sequencing.

If the Universal Adapter Sequence Is 5′-GTCATTGCTTGCAATGTT-3′, Which Universal Primer