Under which circumstance would a researcher most likely use Sanger sequencing instead of high-throughput sequencing?
A) If the researcher is undertaking a comparative genomics study and is sequencing prokaryotes
B) If the researcher is undertaking a comparative genomics study and is sequencing eukaryotes
C) If the researcher is doing a metagenomics study
D) If the researcher is well funded and plans to sequence a large number of organisms
E) If the researcher has only modest funds, lacks miniaturization equipment, and plans to sequence only a relatively small amount

Question

Quizplus · Accepted Answer

Sanger sequencing is a slower and more expensive method than high-throughput sequencing. Therefore, a researcher with modest funds and limited resources would most likely choose Sanger sequencing to sequence only a relatively small amount.

Under Which Circumstance Would a Researcher Most Likely Use Sanger