Sequencing of the DNA from a Chromosome in a Eukaryote

Sequencing of the DNA from a chromosome in a eukaryote

A) does not require separation of the two DNA strands.
B) involves the generation of overlapping fragments of DNA and computer analysis to arrange the fragments in the correct order.
C) is relatively easy because eukaryotic chromosomal DNA is small.
D) can be performed without amplification of DNA.
E) is time consuming because it requires both strands of complementary DNA to be sequenced.