Which DNA sequences would most likely be recognized by a restriction enzyme that cuts at a palindrome? (Note: Complementary strands are shown.)
A) 5ʹ-AGTCGA-3ʹ and 3ʹ-TCAGCT-5ʹ
B) 5ʹ-GTCTGA-3ʹ and 3ʹ-CAGACT-5ʹ
C) 5ʹ-TAAGGT-3ʹ and 3ʹ-ATTCCA-5ʹ
D) 5ʹ-TAGCTA-3ʹ and 3ʹ-ATCGAT-5ʹ
E) 5ʹ-TCGTGGA-3ʹ and 3ʹ-AGCACCT-5ʹ

Question

Quizplus · Accepted Answer

Restriction enzymes recognize specific sequences of nucleotides, which are often palindromic, meaning the sequence reads the same forward and backward on both strands. Choice D (5ʹ-TAGCTA-3ʹ and 3ʹ-ATCGAT-5ʹ) is palindromic, as the sequence on one strand reads the same in the opposite direction on the complementary strand.

Which DNA Sequences Would Most Likely Be Recognized by a Restriction