The sequence 5ʹ-TCATGT-3ʹ is not a restriction enzyme palindromic recognition sequence.What mutational change would most likely make it into one?
A) Changing T to C at the 5ʹ end
B) Changing T to A at the 5ʹ end
C) Changing T to G at the 5ʹ end
D) Changing T to G at the 3ʹ end
E) Changing T to C at the 3ʹ end

Question

Quizplus · Accepted Answer

For a sequence to be a restriction enzyme recognition palindrome, it must read the same on both strands when read in opposite directions. Therefore, changing the first T to an A would make the sequence read 5ʹ-ACATGT-3ʹ on one strand and the complementary strand would read 3ʹ-TGTACA-5ʹ, which is the same when read in the opposite direction.

The Sequence 5ʹ-TCATGT-3ʹ Is Not a Restriction Enzyme Palindromic Recognition