Which activity would be considered cloning of recombinant DNA?
A) Using PCR to produce millions of copies of a specific DNA sequence that came from a human hair for use in forensics
B) Using PCR to produce millions of copies of a specific DNA sequence from a 20,000-year-old wooly mammoth fossil
C) Producing many copies of a gene from a tomato that codes for an enzyme in a yeast cell
D) Using green fluorescent protein as a reporter gene
E) Using a gene that codes for antibiotic resistance as a reporter gene

Question

Quizplus · Accepted Answer

Cloning of recombinant DNA involves the insertion of a specific DNA sequence into a vector, such as a plasmid, to produce many copies. Option C is the only choice that explicitly involves the production of recombinant DNA by inserting a gene from tomato into a yeast cell. Option A involves amplifying a specific DNA sequence but does not involve cloning. Option B involves amplifying a specific DNA sequence from a fossil but does not involve cloning recombinant DNA. Option D and E involve the use of reporter genes but do not involve cloning recombinant DNA.

Which Activity Would Be Considered Cloning of Recombinant DNA