The process of inserting recombinant DNA is considered transfection when
A) the host cells are yeast cells.
B) the host cells are E. coli cells.
C) a selectable marker is used.
D) the vector lacks a replicon.
E) the host cells are mouse tissue culture cells.

Question

Quizplus · Accepted Answer

Transfection specifically refers to the introduction of nucleic acids into eukaryotic cells, such as those from mice or other animals. In contrast, the term transformation is often used when referring to bacterial cells like E. coli. Yeast cells, being eukaryotic, are also typically not referred to in the context of transfection in the same way as mammalian cells. The use of selectable markers or the presence/absence of a replicon in the vector does not change the terminology from transfection to another term; these are aspects of the vector or selection process rather than the method of DNA introduction.

The Process of Inserting Recombinant DNA Is Considered Transfection When