In transgenic experiments researchers often make use of electroporation, screen for reporter genes, and insert vectors into the host cell.In which order would they most likely perform these steps?
A) First electroporation, then screen for reporter genes, then insert vectors
B) First electroporation, then insert vectors, then screen for reporter genes
C) First screen for reporter genes, then electroporation, then insert vectors
D) First screen for reporter genes, then insert vectors, then electroporation
E) First insert vectors, then screen for reporter genes, then electroporation

Question

Quizplus · Accepted Answer

Electroporation involves the delivery of foreign DNA into cells using electric fields, which must be done before any vector insertion. Once the foreign DNA has been inserted, scientist can insert vectors into the host cell. Finally, researchers would screen for reporter genes to identify the cells that have taken up the foreign DNA through electroporation and vector insertion. Therefore, the correct order is first electroporation, then insert vectors, and finally screen for reporter genes.

In Transgenic Experiments Researchers Often Make Use of Electroporation, Screen