Suppose a plasmid has genes that confer resistance to both ampicillin and tetracycline.Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.A cell transformed with the resulting recombinant plasmid should be _______ to ampicillin and _______ to tetracycline.
A) resistant; resistant
B) resistant; sensitive
C) resistant; complementary
D) sensitive; resistant
E) sensitive; complementary

Question

Quizplus · Accepted Answer

Since the foreign DNA is inserted within the ampicillin gene, it disrupts the gene and the cell cannot produce the protein required for resistance to ampicillin. Therefore, the cell will be sensitive to ampicillin. However, the tetracycline resistance gene is still intact, so the cell will remain resistant to tetracycline.

Suppose a Plasmid Has Genes That Confer Resistance to Both