Which statement about genomic and cDNA libraries is true?
A) A genomic library constructed from human brain tissue will be different from a genomic library constructed from human pancreatic tissue.
B) A cDNA library constructed from human brain tissue will be different from a cDNA library constructed from human pancreatic tissue.
C) A cDNA library would be very useful in examining DNA that does not code for mRNA.
D) A genomic library from a tissue will change over time, as opposed to a cDNA library from that same tissue.
E) The construction of a genomic library requires RT-PCR.

Question

Quizplus · Accepted Answer

A cDNA library is constructed from mRNA transcripts present in a tissue, reflecting the genes that are being actively expressed at the time of collection. Since different tissues express different sets of genes (e.g., brain tissue vs. pancreatic tissue), the cDNA libraries from these tissues would be different, capturing the unique expression profiles of each tissue.

Which Statement About Genomic and CDNA Libraries Is True