A restriction enzyme has generated blunt ends.Which event is most likely?
A) The site it recognized is not a palindrome.
B) The enzyme made staggered cuts.
C) It cut in the middle of the palindrome.
D) The fragments it generated had overhangs.
E) The temperature is much above room temperature.

Question

Quizplus · Accepted Answer

Restriction enzymes that generate blunt ends typically cut directly in the middle of their recognition site, which is often a palindromic sequence. This means both strands are cut at the same position, resulting in ends that are flush or 'blunt' rather than staggered, which would produce overhangs.

A Restriction Enzyme Has Generated Blunt Ends