You are performing a recombinant DNA experiment using a restriction enzyme that makes staggered cuts and using the GFP gene as a reporter.The plasmid DNA gets cut properly, but the different pieces do not rejoin properly.Most likely, you have a defective
A) CAS9 protein.
B) restriction enzyme.
C) DNA ligase.
D) GFP.
E) replicon.

Question

Quizplus · Accepted Answer

DNA ligase is responsible for joining the cut pieces of DNA back together, forming recombinant DNA. If the pieces are not joining properly, it is likely due to a defective DNA ligase enzyme. The other choices are not relevant to the specific issue described in the scenario.

You Are Performing a Recombinant DNA Experiment Using a Restriction