The restriction enzymes that are most useful in generating recombinant DNA are those that
A) make random cuts.
B) make staggered cuts.
C) cut in the middle of the palindrome.
D) cut at nonpalindrome sites.
E) do not cut.

Question

Quizplus · Accepted Answer

Restriction enzymes that make staggered cuts, also known as "sticky ends", generate complementary overhangs that can base pair with each other, facilitating the formation of recombinant DNA molecules.

Answer: C 
 Restriction enzymes that cut in the middle of a palindrome, also known as "blunt ends", facilitate the formation of blunt-ended recombinant DNA molecules. However, the efficiency of blunt-end ligation is typically lower than that of sticky-end ligation.

The Restriction Enzymes That Are Most Useful in Generating Recombinant