From the list below, select the sequence of steps for inserting a piece of foreign DNA into a plasmid vector, introducing the plasmid into bacteria, and verifying that the plasmid and the foreign gene are present:
(1) Transform host cells.
(2) Select for the lack of plasmid reporter gene 1 function.
(3) Select for the plasmid reporter gene 2 function.
(4) Digest vector and foreign DNA with a restriction enzyme, which inactivates plasmid reporter gene 1.
(5) Ligate the digested plasmid together with the foreign DNA.
A) 4, 5, 1, 3, 2
B) 4, 5, 1, 2, 3
C) 1, 3, 4, 2, 5
D) 3, 2, 1, 4, 5
E) 1, 3, 2, 5, 4

Question

From the list below, select the sequence of steps for inserting a piece of foreign DNA into a plasmid vector, introducing the plasmid into bacteria, and verifying that the plasmid and the foreign gene are present: 
(1) Transform host cells.
(2) Select for the lack of plasmid reporter gene 1 function.
(3) Select for the plasmid reporter gene 2 function.
(4) Digest vector and foreign DNA with a restriction enzyme, which inactivates plasmid reporter gene 1.
(5) Ligate the digested plasmid together with the foreign DNA.
A) 4, 5, 1, 3, 2
B) 4, 5, 1, 2, 3
C) 1, 3, 4, 2, 5
D) 3, 2, 1, 4, 5
E) 1, 3, 2, 5, 4

Quizplus · Accepted Answer

The correct sequence of steps is:1. Digest vector and foreign DNA with a restriction enzyme, which inactivates plasmid reporter gene 1.2. Ligate the digested plasmid together with the foreign DNA.3. Transform host cells.4. Select for the plasmid reporter gene 2 function.5. Select for the lack of plasmid reporter gene 1 function.

From the List Below, Select the Sequence of Steps for Inserting