Which of the following enzymes would be used to join two DNA fragments?
A) Reverse transcriptase
B) DNA polymerase
C) DNA recombinase
D) DNA endonuclease
E) DNA ligase

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme responsible for joining together two DNA fragments by catalyzing the formation of a phosphodiester bond between the 3'-hydroxyl group at one end of a sugar molecule in one DNA fragment and the 5'-phosphate group at the other end of a sugar molecule in the second DNA fragment. Reverse transcriptase is an enzyme that synthesizes a complementary DNA strand from an RNA template, DNA polymerase is involved in synthesizing new strands of DNA, DNA recombinase is involved in the process of genetic recombination, and DNA endonuclease is an enzyme that cleaves DNA at specific sites.

Which of the Following Enzymes Would Be Used to Join