The two enzymes that are most important in the construction of recombinant DNA are _______ and _______.
A) restriction enzymes; reverse transcriptase
B) restriction enzymes; DNA ligase
C) reverse transcriptase; DNA polymerase
D) TPA; reverse transcriptase
E) cytochrome oxidase; DNA polymerase

Question

Quizplus · Accepted Answer

Restriction enzymes cut DNA at specific sequences, creating "sticky ends" that can pair with complementary sequences on other DNA molecules. DNA ligase then seals the strands together, creating recombinant DNA molecules. Reverse transcriptase and DNA polymerase are also important enzymes in genetic engineering, but they are not the primary enzymes used in constructing recombinant DNA. TPA and cytochrome oxidase are not relevant to genetic engineering.

The Two Enzymes That Are Most Important in the Construction