EcoRI makes staggered cuts when it cleaves DNA, creating single-stranded tails called "sticky ends." These ends can form a complementary base pair. In order for this to happen, which of the following conditions is necessary?
A) The presence of specific helicases
B) High enough temperatures
C) Methyl groups at each end
D) Sufficiently low temperature
E) None of the above

Question

Quizplus · Accepted Answer

In order for the sticky ends to form a complementary base pair, the temperature needs to be sufficiently low to allow for hydrogen bonding between the complementary bases. Helicases and methyl groups do not play a role in this process.

EcoRI Makes Staggered Cuts When It Cleaves DNA, Creating Single-Stranded