The process of inserting recombinant DNA is considered transfection when
A) the host cells are yeast cells.
B) the host cells are E. coli cells.
C) the host cells are mouse tissue culture cells.
D) the vector lacks a replicon.
E) a selectable marker is used.

Question

Quizplus · Accepted Answer

Transfection refers specifically to the process of introducing foreign DNA into eukaryotic cells, such as mouse tissue culture cells. Yeast and E. coli are both prokaryotic cells and the process of introducing foreign DNA into them is usually referred to as transformation. The presence of a replicon or selectable marker are not defining factors for transfection.

The Process of Inserting Recombinant DNA Is Considered Transfection When