Variation at a specific gene is known to affect metabolism in mice, but it is not clear which of the nucleotides are responsible for the changes. In addition to recombinant DNA technology, which the following techniques would be most relevant for revealing which of the nucleotides affect metabolism?
A) Homologous recombination
B) Antisense RNA
C) RNAi
D) Artificial synthesis
E) Mutagenesis

Question

Quizplus · Accepted Answer

Mutagenesis is the process of generating random mutations in a gene of interest to determine the functional significance of each nucleotide. This technique could be used to identify which specific nucleotides are responsible for the changes in metabolism observed in the mice. Homologous recombination, antisense RNA, RNAi, and artificial synthesis are all useful techniques, but they do not directly address the question of which nucleotides are responsible for the metabolic changes.

Variation at a Specific Gene Is Known to Affect Metabolism