cDNA libraries
A) are the same as genomic libraries.
B) include DNA from non-coding sequences.
C) require DNA polymerase to generate.
D) require reverse transcriptase to generate.
E) likely contain all protein-coding genes.

Question

Quizplus · Accepted Answer

cDNA libraries are generated using reverse transcriptase to convert mRNA into cDNA, which can then be cloned into a vector for sequencing or functional studies. Unlike genomic libraries, cDNA libraries only include sequences derived from transcribed mRNA and therefore likely contain all protein-coding genes. Non-coding sequences are not present in cDNA libraries. DNA polymerase is used in the amplification process but not necessary for library generation.

CDNA Libraries