What information would you need to design a synthetic gene for a protein to be expressed (translated) in yeast?
A) Primary structure of the protein and the genetic code
B) Primary structure and secondary structure of the protein and the genetic code
C) Primary structure of the protein, the genetic code, promoter sequence, and codon usage
D) Secondary structure of the protein, the genetic code, and promoter sequence
E) Primary structure of the protein and promoter sequence

Question

Quizplus · Accepted Answer

To design a synthetic gene for a protein to be expressed in yeast, information on the primary structure of the protein is necessary to determine the sequence of nucleotides needed to code for it. The genetic code is also required as it determines which codon corresponds to each amino acid. In addition, the promoter sequence is needed to initiate transcription and the codon usage must also be considered to ensure optimal translation. Therefore, choice C is the best option as it includes all the necessary information.

What Information Would You Need to Design a Synthetic Gene