Which of the following is a disadvantage of shotgun sequencing as compared to hierarchical sequencing?
A) Shotgun sequencing requires a more detailed genetic map.
B) Shotgun sequencing requires the use of bacterial artificial chromosomes.
C) Shotgun sequencing is more expensive.
D) Shotgun sequencing relies more on careful aligning of DNA fragments.
E) None of the above

Question

Quizplus · Accepted Answer

Shotgun sequencing involves randomly breaking the genome into smaller fragments, sequencing them, and then aligning them to create a complete sequence. This process requires careful alignment of DNA fragments, which can be time-consuming and prone to errors. In contrast, hierarchical sequencing involves creating a genetic map of the genome before sequencing, allowing for a more guided approach to sequencing and easier verification of the results. Therefore, D is the correct answer as it states the disadvantage of shotgun sequencing as compared to hierarchical sequencing.

Which of the Following Is a Disadvantage of Shotgun Sequencing