In DNA sequencing, ddNTPs are added at much lower concentrations than are dNTPs. If ddNTPs were added at higher concentrations, what would be the most likely result?
A) The ddNTPs would bind to one another.
B) All of the reactions would terminate early and long strands would not be produced.
C) The reactions would terminate early or late.
D) The ddNTPs would bind to DNA polymerase.
E) The ddNTPs would not bind to the dye.

Question

Quizplus · Accepted Answer

Higher concentrations of ddNTPs would lead to more frequent incorporation into the growing DNA strand, causing premature termination and preventing the formation of long DNA strands.

In DNA Sequencing, DdNTPs Are Added at Much Lower Concentrations