Which of the following would not be required for the purpose of determining the sequence of a 400-base-pair DNA fragment by the standard ddNTP sequencing method?
A) The four dNTPs (dATP, dCTP, dGTP, dTTP)
B) DNA polymerase
C) A short, artificially synthesized primer
D) A bacterial artificial chromosome
E) All of the above would be required.

Question

Quizplus · Accepted Answer

The purpose of determining the sequence of a 400-base-pair DNA fragment by the standard ddNTP sequencing method requires the four dNTPs (dATP, dCTP, dGTP, dTTP), DNA polymerase, and a short, artificially synthesized primer. However, a bacterial artificial chromosome would not be necessary for this purpose as it is a vector used for cloning large DNA fragments (100-300 kilobase pairs) and sequencing an already existing fragment would not require it.

Which of the Following Would Not Be Required for the Purpose