Electrophoresis separates DNA fragments of different sizes, but this technique does not indicate which of the fragments contains the DNA piece of interest. This problem is solved by
A) measuring the sizes of the bands on the gel.
B) removing the bands from the gel and hybridizing them with a known strand of DNA complementary to the gene of interest.
C) knowing the isoelectric points of the piece in question.
D) identifying the molecular weights of the fragments in question.
E) None of the above

Question

Quizplus · Accepted Answer

Removing the bands from the gel and hybridizing them with a known strand of DNA complementary to the gene of interest is a technique called Southern blotting. It allows the visualization of a specific DNA fragment of interest among all the other fragments on the gel. This technique uses a probe that binds specifically to the DNA fragment of interest, allowing its identification.

Electrophoresis Separates DNA Fragments of Different Sizes, but This Technique