Restriction enzymes cleave DNA at specific sequences by hydrolyzing
A) the 3´ hydroxyl of one nucleotide and the 5´ phosphate of the next one.
B) at the 1´ carbons to cleave the nitrogenous bases.
C) at the 2´ carbons to cleave hydroxyl groups.
D) two phosphodiester linkages on the same strand.
E) four phosphodiester linkages, two on each strand.

Question

Quizplus · Accepted Answer

Restriction enzymes cleave DNA by hydrolyzing the phosphodiester bond between the 3´ hydroxyl of one nucleotide and the 5´ phosphate of the next one, resulting in a cut within the DNA strand.

Restriction Enzymes Cleave DNA at Specific Sequences by Hydrolyzing