Treatment of the RNA polymerase/DNA complex with DNase in vitro is a DNA footprinting technique used to:
A) identify the termination sequence for transcription.
B) locate the start site for transcription.
C) locate the promoter site.
D) identify the position of enhancer sequences.
E) none of the above.

Question

Quizplus · Accepted Answer

Treatment of the RNA polymerase/DNA complex with DNase in vitro allows for the identification of the protected regions of DNA where the polymerase is bound. These protected regions correspond to the promoter site, as the polymerase needs to bind to the DNA at the promoter in order to initiate transcription.

Treatment of the RNA Polymerase/DNA Complex with DNase in Vitro