In order for transcription to begin, the DNA-duplex must be "opened" to allow RNA polymerase access to single stranded template. All facilitate "opening" of DNA-duplex EXCEPT: A) low melting temperature, T_m, in the Pribnow box. B) A:T rich DNA duplex. C) negative supercoiling. D) binding of RNA polymerase σ-subunit. E) all are true.

Question

Quizplus · Accepted Answer

All the options listed (low melting temperature in the Pribnow box, A:T rich DNA duplex, negative supercoiling, and binding of RNA polymerase σ-subunit) facilitate the opening of the DNA duplex for transcription to begin, making option E (all are true) the correct answer.

In Order for Transcription to Begin, the DNA-Duplex Must Be