Which of the following is required for RNA splicing to occur?
A) a free 5´ hydroxyl created by hydrolysis of the phosphate ester bond
B) a cyclic phospodiester at the 3´ end of the RNA molecule
C) a 2´ hydroxyl group
D) the enzyme splicase
E) none of the above

Question

Quizplus · Accepted Answer

RNA splicing requires a 2´ hydroxyl group for the reaction to take place. A free 5´ hydroxyl is involved in the ligation step after splicing, and a cyclic phosphodiester at the 2´ end is involved in trans-splicing, not in regular splicing. There is no such enzyme called splicase - the actual enzyme involved in splicing is called a spliceosome.

Which of the Following Is Required for RNA Splicing to Occur