Of the following, which promotes the release of oxygen by hemoglobin? A) increase in [H⁺] B) decrease in [CO₂] C) decrease in [2,3-bisphosphoglycerate] D) Tyr HC2-Val FG5 hydrogen bond breaking E) none of the above

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An increase in [H⁺] (lower pH) promotes the release of oxygen by hemoglobin due to the Bohr effect, where hemoglobin's affinity for oxygen decreases in more acidic conditions.

Of the Following, Which Promotes the Release of Oxygen by Hemoglobin