An important observation that assisted in the elucidation of the secondary structure of DNA was that the mole percent adenine was almost always the same as the mole percent of thymine.

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This is known as Chargaff's rule, and it provided important clues for the double helix structure of DNA, as hydrogen bonding between A and T bases and between C and G bases can account for this observation.

An Important Observation That Assisted in the Elucidation of the Secondary